Document Type : Original Research Article
1 Department of Chemistry, Federal University Gashua, Yobe State, Nigeria
2 Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria
Terpenoids are definite secondary metabolites with active therapeutic components that contribute to plants’ medicinal potential. Strychnos innocua is a Loganiaceae family medicinal plant found in various African countries. This study reports the isolation, characterization, and molecular docking analyses of Linalool (1) and Nerolidol (2) from ethyl acetate root bark extract of S. innocua. Their structures were validated using mass spectrometry, nuclear magnetic resonance (1D and 2D NMR), and compared with literature data. This is a novel report of terpenoids isolated from S. innocua root bark. An in silico docking examination revealed the binding energies of Linalool with the binding sites of Staphylococcus aureus pyruvate carboxylase (PDB: 3HO8) and Pseudomonas aeruginosa virulence factor regulator (PDB: 2OZ6) were -4.7 and –5.6 kcal/mol, respectively. Furthermore, the binding energies of Nerolidol with the binding sites of S. aureus and P. aeruginosa were -5.8 and -6.9 kcal/mol, respectively. Compared to ciprofloxacin (standard drug), which showed binding energies of -6.6 and -8.7 kcal/mol, respectively. This study concluded that Linalool and Nerolidol are abundant in the root bark of S. innocua. At the same time, docking results revealed that the compounds had moderate interactions with S. aureus and P. aeruginosa, exhibiting antibacterial effects.
Terpenoids have found useful therapeutic applications in medicine . While antidysentery, antidiarrhea, antidiabetes, antihypertension, analgesic, anti-inflammatory, antibacterial, antibiotic, antiseptic, and antioxidant activities are some of the biological applications of terpenoids . On the other hand, various additional phytochemicals such as flavonoids, alkaloids, and steroids have also been shown to possess active therapeutic components, which reports show exhibit favorable medicinal properties .
Plants are the primary source of bioactive compounds, and the plant kingdom holds many therapeutic development potentials . Linalool has been demonstrated to possess antibacterial, antifungal, anticancer, anti-inflammatory, and antioxidant activities, while several in vivo studies have proven Linalool’s pharmacological importance . On the other hand, Nerolidol is sesquiterpene alcohol with a flowery odor found in several plants. Researchers have examined its therapeutic properties, which might have favorable influences on human health .
The antimicrobial activity of natural compounds derived from plants has been studied utilizing various in vitro, in vivo, and computational methods. Docking is one of these methods that have received widespread applications in developing antimicrobial drugs . Researchers have also recently employed computational approaches to explore the effects of drugs and vitamins on the human body [8-15]. Ciprofloxacin, a standard antibiotic used to treat various bacterial infections, respiratory tract infections, and urinary tract infections, is significant in treating S. aureus and P. aeruginosa .
Strychnos innocua is a Loganiaceae plant with a straight stem that may reach a height of 18 meters. It has a trunk diameter varying from 7 to 40 cm and many branches. Its leaves are usually simple. However, they have a rounded base in rare instances. S. innocua is found in several countries, including Sudan, Malawi, Cameroon, Uganda, and Nigeria. The plant's root is used to cure gonorrhoea, and a fresh infusion of the plant's root is used to heal snake bites. [17,18]. Kaduna State is one of the Nigerian states where the plant is commonly harvested.
Chemical compositions and antimicrobial evaluations of S. innocua root bark extracts have been studied and published in the literature [19–21]. However, there is a paucity of information concerning the isolation of compounds from S. innocua root bark extracts. This study isolated, characterized and docked two terpenoids, Linalool (1), Nerolidol (2) from the root bark of S. innocua. This is a novel report of Linalool and Nerolidol isolated from the plant’s root bark.
Materials and Methods
Plant collection and extraction
The plant of S. innocua was collected from the wild in Kaduna State, Nigeria, identified and authenticated in the Department of Biological Sciences at ABU, Zaria by Mr. Namadi Sunusi, where V/N – 01884 is the herbarium voucher number. The root bark of S. innocua was dried under shade. Subsequently, it was then crushed to a fine powder. The powder (i.e., pulverized sample, 2 kg) was extracted using the maceration technique with analytical grade solvents, such as n-hexane, ethyl acetate, and methanol, respectively, in increasing polarity, as reported by [19-22].
General experimental procedure
On GC 7890B, MSD 5977A, Agilent Tech, GC-MS of the isolated compounds was performed. Both 1D and 2D NMR spectra were determined on the Varian–Vnmrs 400 MHz spectrometer in ppm (the chemical shift), while chloroform (CdCl3) was the solvent used.
Reagents and chemicals used
The reagents and chemicals used in this study are all analytical grade JDH (methanol, chloroform, ethyl acetate, and n-hexane).
Column chromatography and isolation
Many spots were revealed by thin-layer chromatography (TLC) analysis of the ethyl acetate extract using several solvent systems. The extract (30 g) was combined with 60-120 mesh silica gel and dried before being transferred to a column (size, 5 cm × 60 cm) parked with silica gel and n-hexane. The column was eluted using a suitable solvent system (n-hexane: Ethyl acetate) with increasing polarity (n-hexane 100 %, 9:1, 8:2, 7:3, 6:4, 1:1, 4:6, 3:7, 2:8, 1:9, and 100 % Ethyl acetate) at a flow rate of 1 drop/sec and two hundred and sixty one (261) 50 mL collections. A pre-coated TLC plate was used to monitor these collections with a spraying reagent (Methanol: Glacial Acetic Acid: Sulphuric Acid: P-anisaldehyde at a ratio of 85: 10: 5: 0.l mL), yielding twenty-four fractions (F1-F24). A small amount of F17 was subjected to preparative thin-layer chromatography. The TLC was scraped and placed in a beaker. Chloroform solvent was added, the mixture was filtered, the filtrate was evaporated, and one spot was observed on the TLC plate. This was an isolated Compound 1 (Rf value: 0.24), with a yield value of 32 mg. Furthermore, fractions 8 and 9 were combined to get 2.9 g, mixed with Silica Gel, and left to dry. It was transferred to a column packed with silica gel and eluted with 100 % n-hexane (twenty collections of 10 mL) and a 9:1 n-hexane: Ethyl acetate gradient mixture (forty collections of 10 mL). The flow rate was 1 drop per 5 seconds. The collections were monitored using a pre-coated TLC plate, which yielded eight subfractions (FF1-FF8). Compound 2 was isolated on FF6 using preparative thin-layer chromatography (Rf = 0.23). The yield of 2 was 28 mg.
An in silico docking study was performed on ligands, (isolated compounds and Ciprofloxacin) with target receptors (PDB: 3HO8 and 2OZ6) downloaded from (www.rcsb.org). ChemDraw professional 16.0 was used to generate the ligands two-dimensional (2D) structure, which was then converted to three-dimensional (3D) geometrical optimization using Spartan 20v.1.1/2020. The target receptors in three-dimensional form were prepared using Discovery Studio Visualizer software v.126.96.36.19998, saved in the PDB file format, and uploaded to Pyrx software for docking. The docking output was shown in Discovery Studio with the binding score to examine the protein-ligand interactions [23, 24].
Results and Discussion
Compound 1 (32 mg) was obtained as pale oil. The mass spectrum (Figure 1) of 1 indicated molecular ion peaks at m/z 154 and fragment ions m/z 136, m/z 121, m/z 93, m/z 71, m/z 55, m/z 43, and m/z 27 suggesting its molecular formula to be C10H18O. The NMR spectra data (Table 1) of 1 were very similar to the literature for Linalool with 1H NMR (Figure 2) displaying δH for two allylic methyl protons (δH 1.63 H-8 and 1.76 H-9), two olefinic methine protons (δH 5.19 H-6 and 5.91 H-2), two methylene protons (δH 1.37 H-4 and 2.01 H-5), and one olefinic methylene, methyl and hydroxyl protons (δH 5.33 H-1, 0.90 H-10, and 4.76 -OH). The 13C NMR (Figure 3) and DEPT displayed 10 carbon signals for two methylene groups (δC 22.52 C-5 and 43.87 C-4), two quaternary carbons (δC 75.68 C-3 and 131.96 C-7), three olefinic carbons (δC 108.65 C-6, 120.17 C-1, and 145.25 C-2), and three methyl groups (δC 17.53 C-8, 24.95 C-9, and δC 29.83 C-10).
Compound 2 (28 mg) was obtained as a pale yellow oil. The mass spectrum (Figure 4) of 2 indicated fragment ion peaks at m/z 204, representing a loss of H2O from molecular ions at m/z 222, and fragment ions m/z 189, m/z 175, m/z 161, m/z 136, m/z 121, m/z 107, m/z 93, m/z 69, m/z 55, and m/z 41 suggesting its molecular formula to be C15H26O. The NMR spectra data (Table 2) of 2 were very similar to the literature for Nerolidol with 1H NMR (Figure 5), displaying δH for four methylene protons (δH 1.36 H-4, 2.00 H-5, 2.33 H-8, and 2.35 H-9), one olefinic methylene protons (δH 5.40 H-1), three allylic methyls (δH 1.50 H-12, 1.60 H-14, and 1.73 H-13), one methyl protons (δH 0.94 H-15), three olefinic methine protons (δH 5.15 H-6, 5.15 H-10, and 6.00 H-2), one hydroxyl proton (δH 4.54 -OH) The 13C NMR (Figure 6) and DEPT displayed 15 carbon signals for three quaternary carbons (δC 73.72 C-3, 133.45 C-11, and 135.67 C-7), three allylic methyl carbons (δC 15.49 C-14, 17.46 C-13, and δC 25.21 C-12), one methyl carbon (δC 29.31 C-15), Four methylene carbons (δC 22.91 C-5, 27.43 C-9, 39.38 C-8, and 43.10 C-4), three olefinic methine carbons (δC 122.53 C-10, δC 124.75 C-6, and 145.44 C-2), and one olefinic methylene (δC 110.54 C-1).
Figure 1. Mass Spectrum for Linalool
Table 1. The NMR (400 MHz) Data of Linalool
Figure 2. 1H NMR Spectrum for Linalool
Figure 3. 13C NMR Spectrum for Linalool
Figure 4. Mass Spectrum for Nerolidol
Table 2. The NMR (400 MHz) Data of Nerolidol
Figure 5. 1H NMR Spectrum for Nerolidol
Figure 6. 13C NMR Spectrum for Nerolidol
The presence of terpenoids was verified in the phytochemical analysis of the ethyl acetate extract, and the extract demonstrated in vitro antibacterial activity against S. aureus, B. subtilis, and P. aeruginosa . This bolsters the therapeutic value of medicinal plants [27-29]. Linalool and Nerolidol were isolated from the extract using chromatographic separation, and their structures were identified using spectroscopic analysis and compared to previously published data [30,31].
Linalool is an acyclic monoterpenoid found in many plant species . It is also a component of essential oils in various plant species and has demonstrated improved antibacterial action against Salmonella typhimurium in nanoemulsions . Nerolidol, commonly known as peruviol, is sesquiterpenoid alcohol with antibacterial, antioxidant, and other pharmacological effects. The antibiofilm properties of Nerolidol were also studied, with the findings indicating its therapeutic efficacy .
The compounds' interactions with the target receptors (PDB: 3HO8 and 2OZ6) were investigated using molecular docking and compared to ciprofloxacin (standard drug). Table 3 reveals that the binding scores of the compounds with S. aureus pyruvate carboxylase 3HO8 (receptor) are all moderately comparable to ciprofloxacin. Though the binding energy of Nerolidol (-5.8 kcal/mol) is higher than that of Linalool (-4.7 kcal/mol), their interactions with the receptor are shown in Figures 7 and 8, respectively. Ciprofloxacin's binding energy was -6.6 kcal/mol, and its interaction is shown in Figure 9.
Table 4 shows that Linalool and Nerolidol binding scores with the P. aeruginosa virulence factor regulator 2OZ6 (receptor) are also moderately comparable to ciprofloxacin. Nerolidol has higher binding energy (-6.9 kcal/mol) than Linalool (-5.6 kcal/mol). Their interactions with the receptor are shown in Figures 10 and 11, respectively. Ciprofloxacin's binding energy was -8.7 kcal/mol, and its interaction with the receptor is shown in Figure 12.
Table 3. Binding Energy Results of the Isolated Compounds/Ciprofloxacin with Target Receptor (PDB: 3HO8)
Table 4. Docking Results of the Isolated Compounds/Ciprofloxacin with Target Receptor (PDB: 2OZ6)
Figure 9. 2D Interaction of Ciprofloxacin with crystal structure of S. aureus (PDB: 3HO8)
Figure 10. 2D Interaction of Linalool with crystal structure of P. aeruginosa (PDB: 2OZ6)
Figure 11. 2D Interaction of Nerolidol with crystal structure of P. aeruginosa (PDB: 2OZ6)
Figure 12. 2D Interaction of Ciprofloxacin with crystal structure of S. aureus (PDB: 20Z6)
Column chromatography was utilized to isolate two compounds (Linalool and Nerolidol) from S. innocua root bark, and their structures were determined using MS and NMR spectroscopy. Linalool and Nerolidol demonstrated moderate binding scores (-4.7 and -5.8 kcal/mol respectively) with the binding sites of S. aureus (PDB: 3HO8) compared to Ciprofloxacin (-6.6 kcal/mol). Furthermore, their binding energies of -5.6 and -6.9 kcal/mol with P. aeruginosa (PDB: 2OZ6), were also comparable to ciprofloxacin (-8.7 kcal/mol). These moderate binding energies, found that Linalool and Nerolidol might be potential antibacterial agents.
The authors acknowledge Mr. Silas Ekwuribe of the Multi-User Laboratory, Chemistry Department at Ahmadu Bello University Zaria, Nigeria.
The authors declare no competing interests.
Ahmed Jibrin Uttu : 0000-0002-4089-5529