Document Type : Original Research Article


Department of Physical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, P.O.Box 65174, Hamedan, Iran



A new and easy to fabricate voltammetric biosensor for acetaminophen determination was developed based on horseradish peroxidase (HRP) trapped between silica sol-gel film and multi-walled carbon nanotubes on glassy carbon electrode. Acetaminophen determinations were carried out in presence of H2O2 as enzyme activator. The modified electrode showed excellent electrocatalytic activity and rapid response to acetaminophen in the presence of H2O2 as enzyme activator. Various parameters influencing the biosensor performance such as amount of enzyme, H2O2 concentration, potential scan rate and pH have been investigated. Under the optimal conditions, a wide linear range of 1.85×10−6 to 2.7×10−3 M for acetaminophen determination was obtained. Limit of detection was calculated about 18 nM and sensitivity was about 220 nA/µM. Furthermore, the proposed biosensor was successfully examined for simultaneous determination of acetaminophen with uric acid (UA) and folic acid (FA) as prevalent interferes. The proposed biosensor showed satisfactory stability for 3 weeks and applicability of developed biosensor was confirmed with accurately evaluation of acetaminophen in real samples such as urine and tablet.

Graphical Abstract

Determination of acetaminophen using a glassy carbon electrode modified by horseradish peroxidase trapped in MWCNTs/silica sol-gel matrix


Main Subjects

[1]Fanjul-Bolado P, Lamas-Ardisana J, Hernández-Santos D and Costa-García A 2009. Anal. Chim. Acta638 133-8
[2] Bui M-N, Li A, Han N, Pham X-H and Seong H. 2012 Sens. Actuators B174 318-24
[3] Shrestha R and Pradhananga R 2009. J. Nepal Chem. Soc.24 39-44
[4]Gioia G, Andreatta P, Boschetti S and Gatti R 2008 J. Pharm. Biomed. Anal.48 331-9
[5]Eustaquio A, Blanco M, Jee R and Moffat A 1999 Anal. Chim. Acta383 283-90
[6]Sanghavi J and Srivastava K 2010 Electrochim. Acta55 8638-48
[7]Behera S, Ghanty S, Ahmad F, Santra S and Banerjee S 2012 J. Anal. Bioanal. Tech.3 2-6
[8]Bouhsain Z, Garrigues S and de la Guardia M 1996. Analyst121 1935-8
[9] Lima B, Torres M, Guimarães F, Verly M, Silva L M D, CarvalhoJúnior Á D and dos Santos W T 2014 J. Braz. Chem. Soc.25 478-83
[10]Bahramipur H and Jalali F 2012 Afr. J. Pharm. Pharmacol.6 1298-305
[11] Wang S-F, Xie F and Hu R-F 2007 Sens. Actuators B123 495-500
[12] Radovan C, Cofan C and Cinghita D 2008 Electroanalysis 20 1346-53
[13]Jia L, Zhang X-H, Li Q and Wang S-F 2007 J. Anal. Chem.62 266-269
[14] Felix F S, Brett C and Angnes L 2007 J. Pharm. Biomed. Anal.43 1622-1627
[15]Sima V, Cristea C, Lăpăduş F, Marian I, Marian A and Săndulescu R 2008 J. Pharm. Biomed. Anal.48 1195-200
[16]Xu Z, Yue Q, Zhuang Z and Xiao D 2009. Microchim. Acta.164 387-93
[17] ShangGuan X, Zhang H and Zheng J 2008 Anal. Bioanal.Chem.391 1049-55
[18] Li M and Jing L 2007 Electrochim. Acta52 3250-3257
[19] Wang J, Gu M, Di J, Gao Y, Wu Y and Tu Y 2007. Bioprocess. Biosyst. Eng.30 289-296
[20] Ghodsi J, Rafati A A, Shoja Y, Najafi M 2015 J. Electrochem. Soc. 162 B69-B74
[21] Ghodsi J, Rafati A A, Shoja Y 2016 Sens. Actuators B. 224 692-699
[22] Li Y, Qin C, Chen C, Fu Y, Ma M and Xie Q 2012 Sens. Actuators B 168 46-53
[23] Wang B and Dong S 2000 J. Electroanal. Chem.487 45-50
[24] Wang Y, Zhang X, Chen Y, Xu H, Tan Y and Wang S 2010 Am. J. Biomed. Sci.2 209-216
[25] Chen L, Gu B, Zhu G, Wu Y, Liu S and Xu C 2008 J. Electroanal. Chem.617 7-13
[26] Zhang Y, Zhang J, Wu H, Guo S and Zhang J 2012 J. Electroanal. Chem.681 49-55
[27] Huang S, Qu Y, Li R, Shen J and Zhu L 2008 Microchim. Acta162 261-268
[28]Azevedo A M, Martins V C, Prazeres D M, Vojinović V, Cabral J and Fonseca L P 2003 Biotechnol. Annu. Rev.9 199-247
[29] Ruzgas T, Csöregi E, Emnéus J, Gorton L and Marko-Varga G 1996 Anal. Chim. Acta330 123-138
[30]Bogdanovskay V, Fridman V, Tarasevich M and Scheller F 1994. Anal. Lett.27 2823-2847
[31]Qian L and Yang X 2006. Talanta68 721-727
[32]Morrin A, Wilbeer F, Ngamna O, Moulton S E, Killard A J, Wallace G G and Smyth M R 2005 Electrochem. Commun.7 317-322
[33]Ledru S and Boujtita M 2004. Bioelectrochem. 64 71-78
[34]Mazloum-Ardakani M, Sabaghian F, Khoshroo A and Naeimi H 2014. Chin. J. Catal.35 565-572
[35]Benvidi A, Dehghani-Firouzabadi A, Mazloum-Ardakani M, Mirjalili B-B F and Zare R 2015. J. Electroanal. Chem.736 22-29
[36]Abdelwahab A A and Shim Y-B 2015. Sens. Actuators B221 659-665
[37] Chen X, Zhu J, Xi Q and Yang W 2012. Sens. Actuators B161 648-654
[38] Kung C-W, Lin C-Y, Vittal R and Ho K-C 2013. Sens. Actuators B182 429-438
[39]Haghshenas E, Madrakian T and Afkhami A 2015 Mat. Sci. Eng. C57 205-214
[40] Liu B, Ouyang X, Ding Y, Luo L, Xu D and Ning Y 2016. Talanta146 114-121
[41] Liu G-T, Chen H-F, Lin G-M, Ye P-p, Wang X-P, Jiao Y-Z, Guo X-Y, Wen Y and Yang H-F 2014. Biosens. Bioelectron.56 26-32
[42]Xu C-X, Huang K-J, Fan Y, Wu Z-W and Li J 2012 J. Mol. Liq.165 32-37