Advanced Journal of Chemistry, Section A
5.9(Q2)
CiteScore
31
h-index

Uncovering Male Fertility–Modulating Potential of Murraya koenigii through Network Pharmacology and In Silico Targeting of Reproductive Proteins

Articles in Press

Document Type : Original Research Article

Authors

Nizan Mauyah 1
Rajuddin Rajuddin 2
Suhartono Suhartono 3
Hamny Sofyan 4
Mulkan Azhary 5

1 Doctoral Program in Medical Sciences, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia

2 Deparment of Obstetrics and Gynecology Medicine, Faculty of Medicine, Syiah Kuala University, Banda Aceh, Indonesia

3 Departement of Biology, Faculty of Mathematic and Natural Sciences, Universitas Syiah Kuala, Aceh 23111, Indonesia

4 Laboratory of Anatomy, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, 23111 Indonesia

5 Department of Anatomy and Histology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia

10.48309/ajca.2026.565726.2004
Abstract
Murraya koenigii (L.) Spreng., commonly known as curry leaf, is widely utilized in traditional medicine and valued for its richness in bioactive compounds, including carbazole alkaloids, flavonoids, tannins, and essential oils. While its antioxidant, anti-inflammatory, and metabolic regulatory activities are well established, its potential role in reproductive biology remains insufficiently studied. This study aimed to investigate the potential of M. koenigii phytochemicals to modulate male fertility by targeting key reproductive proteins and receptors. Network pharmacology and molecular docking analyses were employed to examine interactions with gonadotropin-releasing hormone receptor (GNRHR), follicle-stimulating hormone receptor (FSHR), cAMP-responsive element modulator (CREM), and protamine 2 (PRM2). The constructed interaction network revealed biologically relevant associations, particularly the CREM–PRM2 regulatory axis, which is crucial for spermatogenesis and sperm chromatin remodeling. Docking analyses suggested that selected phytoconstituents possess favorable binding affinities toward gonadotropin-related receptors, supporting their potential to influence the hypothalamic–pituitary–gonadal axis and downstream transcriptional events. These findings provide mechanistic insights into the traditional use of M. koenigii in reproductive health and highlight its promise as a natural source for developing botanical-based therapeutics in male infertility management.

Graphical Abstract

Uncovering Male Fertility–Modulating Potential of Murraya koenigii through Network Pharmacology and In Silico Targeting of Reproductive Proteins

Keywords

Murraya koenigii
Phytochemicals
Male fertility
Spermatogenesis
CREM
FSHR

Subjects

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Advanced Journal of Chemistry, Section A

Articles in Press, Accepted Manuscript
Available Online from 09 February 2026

  • Receive Date 16 December 2025
  • Revise Date 12 January 2026
  • Accept Date 06 February 2026

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