Determination of Acetaminophen using a Glassy Carbon Electrode Modified by Horseradish Peroxidase Trapped in MWCNTs/Silica Sol-Gel Matrix

Ghodsi, Javad; Rafati, Amir Abbas; Shoja, Yalda

doi:10.29088/sami/AJCA.2018.5.3955

Document Type : Original Research Article

Authors

Department of Physical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, P.O.Box 65174, Hamedan, Iran

10.29088/sami/AJCA.2018.5.3955

Abstract

A new and easy to fabricate voltammetric biosensor for acetaminophen determination was developed based on horseradish peroxidase (HRP) trapped between silica sol-gel film and multi-walled carbon nanotubes on glassy carbon electrode. Acetaminophen determinations were carried out in presence of H2O2 as enzyme activator. The modified electrode showed excellent electrocatalytic activity and rapid response to acetaminophen in the presence of H2O2 as enzyme activator. Various parameters influencing the biosensor performance such as amount of enzyme, H2O2 concentration, potential scan rate and pH have been investigated. Under the optimal conditions, a wide linear range of 1.85×10−6 to 2.7×10−3 M for acetaminophen determination was obtained. Limit of detection was calculated about 18 nM and sensitivity was about 220 nA/µM. Furthermore, the proposed biosensor was successfully examined for simultaneous determination of acetaminophen with uric acid (UA) and folic acid (FA) as prevalent interferes. The proposed biosensor showed satisfactory stability for 3 weeks and applicability of developed biosensor was confirmed with accurately evaluation of acetaminophen in real samples such as urine and tablet.

Graphical Abstract

Keywords

Main Subjects

Analytical chemistry

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Advanced Journal of Chemistry, Section A

Determination of Acetaminophen using a Glassy Carbon Electrode Modified by Horseradish Peroxidase Trapped in MWCNTs/Silica Sol-Gel Matrix

References

References

Volume 1, Issue 1
September 2018
Pages 39-55

Determination of Acetaminophen using a Glassy Carbon Electrode Modified by Horseradish Peroxidase Trapped in MWCNTs/Silica Sol-Gel Matrix

References

References

Volume 1, Issue 1September 2018Pages 39-55

Volume 1, Issue 1
September 2018
Pages 39-55