5.9(Q2)
CiteScore
31
h-index

Enhanced Trypan Blue Removal from Wastewater using Surface-Modified MOF-5 Adsorbents

Document Type : Original Research Article

Authors

1 Chemistry Department, Faculty of Science, Al-Manar University, Tunisia, Tunisia

2 Laboratory of Water, Membranes and Environmental Biotechnology, Water Researches and Technologies Center, BP 273, Soliman 8020, Tunisia

10.48309/ajca.2026.562486.1983
Abstract
The present study shows the synthesis of porous materials MOF-5 via a solvothermal method for wastewater treatment, particularly in the processes of capturing and removing Trypan Blue (TB) dye from water. The structural and morphological properties of the synthesized MOF-5 were characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and thermogravimetric analysis (TGA). At optimized conditions (pH = 3, T = 25 °C), MOF-5 demonstrated a maximum TB removal efficiency of 77%. Adsorption isotherm studies indicated that the process was best described by the Langmuir model, suggesting monolayer adsorption with a calculated maximum adsorption capacity (Qmax) of 25 mg/g. Kinetic studies showed that the adsorption followed a pseudo-second-order model. Furthermore, thermodynamic parameters confirmed that the adsorption process was spontaneous and endothermic. These findings demonstrate that MOF-5 is a promising adsorbent for dye removal from wastewater, with potential for scaling up from laboratory to industrial applications.

Graphical Abstract

Enhanced Trypan Blue Removal from Wastewater using Surface-Modified MOF-5 Adsorbents

Keywords

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[1] Katheresan, V., Kansedo, J., Lau, S.Y. Efficiency of various recent wastewater dye removal methods: A review. Journal of Environmental Chemical Engineering, 2018, 6(4), 4676–4697.
[2] Benkhaya, S., M'rabet, S., El Harfi, A. Classifications, properties, recent synthesis and applications of azo dyes. Heliyon, 2020, 6(1), e03271.
[3] Chemingui, H., Rezma, S., Lafi, R., Alhalili, Z., Missaoui, T., Harbi, I., Smiri, M., Hafiane, A. Investigation of methylene blue adsorption from aqueous solution onto ZnO nanoparticles: Equilibrium and Box-behnken optimisation design. International Journal of Environmental Analytical Chemistry, 2023, 103(12), 2716–2741.
[4] Beydaghdari, M., Saboor, F.H., Babapoor, A., Asgari, M. Recent progress in adsorptive removal of water pollutants by metal‐organic frameworks. ChemNanoMat, 2022, 8(2), e202100400.
[5] Djama, C., Bouguettoucha, A., Chebli, D., Amrane, A., Tahraoui, H., Zhang, J., Mouni, L. Experimental and theoretical study of methylene blue adsorption on a new raw material, cynara scolymus—A statistical physics assessment. Sustainability, 2023, 15(13), 10364.
[6] Kenawy, E.-R., Ghfar, A.A., Wabaidur, S.M., Khan, M.A., Siddiqui, M.R., Alothman, Z.A., Alqadami, A.A., Hamid, M. Cetyltrimethylammonium bromide intercalated and branched polyhydroxystyrene functionalized montmorillonite clay to sequester cationic dyes. Journal of Environmental Management, 2018, 219, 285–293.
[7] Beltrán, E.M., Pablos, M.V., Torija, C.F., Porcel, M.A., González-Doncel, M. Uptake of atenolol, carbamazepine and triclosan by crops irrigated with reclaimed water in a mediterranean scenario. Ecotoxicology and Environmental Safety, 2020, 191, 110171.
[8] Adnan, M., Xiao, B., Ali, M.U., Xiao, P., Zhao, P., Wang, H., Bibi, S. Heavy metals pollution from smelting activities: A threat to soil and groundwater. Ecotoxicology and Environmental Safety, 2024, 274, 116189.
[9] Cai, Z., Deng, X., Wang, Q., Lai, J., Xie, H., Chen, Y., Huang, B., Lin, G. Core-shell granular activated carbon and its adsorption of trypan blue. Journal of Cleaner Production, 2020, 242, 118496.
[10] Kaur, H., Devi, N., Siwal, S.S., Alsanie, W.F., Thakur, M.K., Thakur, V.K. Metal–organic framework-based materials for wastewater treatment: Superior adsorbent materials for the removal of hazardous pollutants. ACS Omega, 2023, 8(10), 9004–9030.
[11] Makhloufi, K., Samb, I., Srarfi, F. Clay-based ceramic membranes. Advanced Ceramic Materials- Emerging Technologies, 2024, 3049- 8856.
[12] Mechi, L., Chemingui, H., Chékir, J., Alsukaibi, A.K., Azaza, H., Mhiri, M. Plant mediated green synthesis of zinc oxide nanoparticles for photocatalytic degradation of trypan blue: Optimisation via box-behnken design. Results in Chemistry, 2025, 18, 102822.
[13] Hu, Q., Hao, L. Adsorption technologies in wastewater treatment processes. Water, 2025, 17(15), 2335.
[14] Khedidja, M., Bechir, M., Feyda, S., Ali, T.M. Development of ceramic filtering membranes based on tunisian clay and phosphate sludge in wastewater treatment. Solid State Sciences, 2024, 148, 107430.
[15] Priyadarshini, B., Patra, T., Sahoo, T.R. An efficient and comparative adsorption of congo red and trypan blue dyes on MgO nanoparticles: Kinetics, thermodynamics and isotherm studies. Journal of Magnesium and Alloys, 2021, 9(2), 478–488.
[16] El-Idreesy, T.T., Khoshala, O., Firouzi, A., Elazab, H.A. Equilibrium and Kinetic Study on the Biosorption of Trypan Blue from Aqueous Solutions using Avocado Seed Powder. Biointerface Research in Applied Chemistry, 2021, 11(3), 11042 – 11053
[17] Aracagök, Y.D. An investigation of the trypan blue dye's biosorption on fungal biomass. Gümüshane Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2023, 13(3), 605-615.
[18] Chemingui, H., Missaoui, T., Mzali, J.C., Yildiz, T., Konyar, M., Smiri, M., Saidi, N., Hafiane, A., Yatmaz, H. Facile green synthesis of zinc oxide nanoparticles (ZnO NPs): Antibacterial and photocatalytic activities. Materials Research Express, 2019, 6(10), 1050b1054.
[19] Sriram, G., Baby, N., Dhanabalan, K., Arunpandian, M., Selvakumar, K., Sadhasivam, T., Oh, T.H. Studies of various batch adsorption parameters for the removal of trypan blue using Ni-Zn-Bi-layered triple hydroxide and their isotherm, kinetics, and removal mechanism. Inorganics, 2024, 12(11), 296.
[21] Zhu, R., Chen, Q., Zhou, Q., Xi, Y., Zhu, J., He, H. Adsorbents based on montmorillonite for contaminant removal from water: A review. Applied Clay Science, 2016, 123, 239–258.
[22] Pouramini, Z., Mousavi, S.M., Babapoor, A., Hashemi, S.A., Lai, C.W., Mazaheri, Y., Chiang, W.-H. Effect of metal atom in zeolitic imidazolate frameworks (ZIF-8 & 67) for removal of dyes and antibiotics from wastewater: A review. Catalysts, 2023, 13(1), 155.
[23] Soltani, Z., Hoseinzadeh, M., Saboor, F.H. Biomass gasification process enhancing using metal-organic frameworks. Advanced Journal of Chemistry, Section A, 2024, 7(1), 89–109.
[24] Shams, P., Panahi, H., Tahan, M., Jahansooz, F., Heidaripour, A. Fabrication of curcumin (CCM) delivery system based on ZIF-8 nanoparticles (ZIF-8@ CCM) with anticancer application. Journal of Medicinal and Nanomaterials Chemistry, 2023, 5(4), 267-275.
[25] Sajjadnejad, M., Haghshenas, S.M.S. Metal organic frameworks (MOFs) and their application as photocatalysts: Part II characterization and photocatalytic behavior. Advanced Journal of Chemistry, Section A, 2023, 6(2), 172–187.
[26] Petit, C., Bandosz, T.J. Enhanced adsorption of ammonia on metal‐organic framework/graphite oxide composites: Analysis of surface interactions. Advanced Functional Materials, 2010, 20(1), 111–118.
[27] Mohammadi, A.A., Moghanlo, S., Kazemi, M.S., Nazari, S., Ghadiri, S.K., Saleh, H.N., Sillanpää, M. Comparative removal of hazardous cationic dyes by MOF-5 and modified graphene oxide. Scientific Reports, 2022, 12(1), 15314.
[28] Mohammed, M.T.E., Djamel, N., Mohamed, T., Amokrane, S. Study of the adsorption of an organic pollutant onto a microporous metal organic framework. Water Science and Technology, 2021, 83(1), 137–151.
[29] Kumar, G., Masram, D.T. Sustainable synthesis of MOF-5@ GO nanocomposites for efficient removal of rhodamine B from water. ACS Omega, 2021, 6(14), 9587–9599.
[30] Saeed-Ul-Hassan, M., Ehtisham, M., Badawi, A.K., Khan, A.M., Khan, R.A., Ismail, B. A comparative study of moisture adsorption on GO, mof-5, and GO/MOF-5 composite for applications in atmospheric water harvesting. Nanoscale Advances, 2024, 6(14), 3668–3679.
[31] Zhang, S., Ding, J., Tian, D., Su, W., Liu, F., Li, Q., Lu, M. Preparation of novel poly (sodium p-styrenesulfonate)/sodium alginate hydrogel incorporated with MOF-5 nanoparticles for the adsorption of Pb (II) and tetracycline. Journal of Molecular Structure, 2024, 1300, 137313.
[32] Asadevi, H., Prasannakumaran Nair Chandrika Kumari, P., Padmavati Amma, R., Khadar, S.A., Charivumvasathu Sasi, S., Raghunandan, R. ZnO@ MOF-5 as a fluorescence “turn-off” sensor for ultrasensitive detection as well as probing of copper (II) ions. ACS Omega, 2022, 7(15), 13031–13041.
[33] Chemingui, H., Riahi, R., Salem, W.B., Dbouba, H., Bensacia, N., Hannechi, A. Modified ceratonia siliqua as low-cost biosorbent for paracetamol removal: Equilibrium study and optimization via box-behnken design. Biomass Conversion and Biorefinery, 2025, 15, 17887–17904.
[34] Chemingui, H., Lafi, R., Missaoui, T., Montasser, I., Hafiane, A., Kamoun, M. Eco-friendly approach for the synthesis of zinc oxide nanoparticles (ZnO NPs) using verbena officinalis l: Enhancement of photocatalytic activity under UV light and application of response surface methodology. Biomass Conversion and Biorefinery, 2025, 15(3), 4281–4299.
[36] Siyal, A.A., Shamsuddin, R., Low, A., Hidayat, A. Adsorption kinetics, isotherms, and thermodynamics of removal of anionic surfactant from aqueous solution using fly ash. Water, Air, & Soil Pollution, 2020, 231(10), 509.
[37] Talha, K., Wang, B., Liu, J.-H., Ullah, R., Feng, F., Yu, J., Chen, S., Li, J.-R. Effective adsorption of metronidazole antibiotic from water with a stable Zr (IV)-MOFs: Insights from DFT, kinetics and thermodynamics studies. Journal of Environmental Chemical Engineering, 2020, 8(1), 103642.
[38] Kanmaz, N., Yardimci, B., Demircivi, P. In situ synthesis of MIL-125 on cinnamon stick and improved via carboxymethyl cellulose: A sustainable approach for super-high crystal violet adsorption. Journal of Colloid and Interface Science, 2025, 678, 366–377.
[39] Gupta, H., Saini, I., Singh, V., Singh, V., Yarramaneni, S., Grover, P. Fast decomposition of organic contaminant in wastewater using Zn and Mn bimetallic metal organic frameworks. Polyhedron, 2024, 261, 117116.
[40] Kumar, G., Masram, D.T. Sustainable synthesis of MOF-5@ GO nanocomposites for efficient removal of rhodamine b from water. ACS Omega, 2021, 6(14), 9587–9599.
[41] Zhang, N., Li, H., Xu, Z., Yuan, R., Xu, Y., Cui, Y. Enhanced acetone sensing property of a sacrificial template based on cubic-like MOF-5 doped by Ni nanoparticles. Nanomaterials, 2020, 10(2), 386.
[42] Harisankar, A., Preethi, P., Sreeja, T., Rejani, P., Murali, M., Raghunandan, R. Zinc oxide functionalized MOF-5 for the adsorptive removal of Pb (II) metal ions and photocatalytic degradation of methylene blue dye in aqueous medium. Ionics, 2024, 30(4), 2313–2331.
[43] Aberrwaila, H., Chemingui, H., Hafiane, A., Kamoun, M. Comparative efficiency of ZIF‐67 and ZIF‐67@ ZnO nanocomposites for trypan blue adsorption: Preparation and RSM modeling. Nano Select, 2025, e70083.
[44] Shiri, M., Hosseinzadeh, M., Shiri, S., Javanshir, S. Adsorbent based on MOF-5/cellulose aerogel composite for adsorption of organic dyes from wastewater. Scientific Reports, 2024, 14(1), 15623.
[45] Edoamodu, C.E., Nwodo, U.U. Decolourization of synthetic dyes by laccase produced from bacillus sp. NU2. Biotechnology & Biotechnological Equipment, 2022, 36(1), 95–106.
[46] Chemingui, H., Kahloul, M., El Abed, B., Ben Amor, T., Hafiane, A. Green synthesis of zinc oxide nanoparticles using albizia procera leaf extract: Degradation of methylene blue dye via advanced oxidation process and box–behnken design. Clean Technologies and Environmental Policy, 2024, 26(10), 3273–3295.
[47] Amari, A., Alawameleh, H.S.K., Isam, M., Maktoof, M.A.J., Osman, H., Panneerselvam, B., Thomas, M. Thermodynamic investigation and study of kinetics and mass transfer mechanisms of oily wastewater adsorption on UIO-66–MnFe2O4 as a metal–organic framework (MOF). Sustainability, 2023, 15(3), 2488.
[48] Nazir, M.A., Bashir, M.S., Jamshaid, M., Anum, A., Najam, T., Shahzad, K., Imran, M., Shah, S.S.A., ur Rehman, A. Synthesis of porous secondary metal-doped MOFs for removal of rhodamine B from water: Role of secondary metal on efficiency and kinetics. Surfaces and Interfaces, 2021, 25, 101261.
[49] Zhong, X., Lu, Z., Liang, W., Hu, B. The magnetic covalent organic framework as a platform for high-performance extraction of Cr (VI) and bisphenol a from aqueous solution. Journal Of Hazardous Materials, 2020, 393, 122353.
[51] Nadaroglu, H., Cicek, S., Gungor, A.A. Removing trypan blue dye using nano-Zn modified luffa sponge. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2017, 172, 2–8.
[52] Wang, X., Liu, Z., Ye, X., Hu, K., Zhong, H., Yu, J., Jin, M., Guo, Z. A facile one-step approach to functionalized graphene oxide-based hydrogels used as effective adsorbents toward anionic dyes. Applied Surface Science, 2014, 308, 82–90.
[53] Li, H., Eddaoudi, M., O'Keeffe, M., Yaghi, O.M. Design and synthesis of an exceptionally stable and highly porous metal-organic framework. Nature, 1999, 402(6759), 276–279.
[54] Aziz, M., Hassan, R., Saeed-Ul-Hassan, M., Ehtisham, M., Almatawa, M.S., Badawi, A.K., Ismail, B. Efficient water capture under low humidity using Ni-modified MOF-5: Scalable atmospheric water harvesting systems. RSC Advances, 2025, 15(41), 34003–34015.

Articles in Press, Accepted Manuscript
Available Online from 19 February 2026

  • Receive Date 27 November 2025
  • Revise Date 07 January 2026
  • Accept Date 15 February 2026